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2 Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand

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Collagen peptides from discarded cowhide, exhibiting the highest DPP-IV inhibitory activity (IC50 of 3.04 0.13 mg/mL), were obtained through double enzymatic hydrolysis (papain and compound protease) combined with optimized ultrasound-assisted ionic liquids pretreatment, which exposed active sites, enhanced hydrophobic amino acids, and improved gastrointestinal stability [75]